WebFor further information on cysteine bioconjugation methods, readers are referred to other reviews in this area. 8–10 The latter, lysine, has been extensively studied in many …

A series of selective events Nature Reviews Chemistry

WebCysteine-targeted BMIE modification (>90% yield at pH 8.0) of a model protein, the S203C variant of carboxypeptidase G2 (CPG2), measured with ESI-MS, confirms its utility as a site-selective bioconjugation method. ICP-MS analysis confirms mono-metallation of the BMIE-modified CPG2 protein with Zn ++, Cu ++, and Co ++. WebMost significantly, the reaction generates succinimides 1 that are unstable over prolonged time in vivo, undergoing retro-conjugate additions and subsequent trapping with endogenous thiols such as glutathione. 10–13 To overcome this, the succinimides must be hydrolysed post-conjugation, which can lead to a reduction in the yield of the … citizeship green card requirements courses

One-pot thiol–amine bioconjugation to maleimides: simultaneous ...

WebThis protocol is based on a thiol-Michael-type addition of native or engineered cysteine residues to carbonylacrylic reagents equipped with functional compounds such as cytotoxic drugs. This approach is a robust alternative to the conventional maleimide technique; the reaction is irreversible and uses synthetically accessible reagents. WebJan 4, 2024 · Cysteine (Cys)-specific bioconjugation has widespread applications in the synthesis of protein conjugates, particularly for the functionalization of antibodies. Here, we report the discovery of trans-styryl sulfonyl fluoride (SSF) as a near-perfect Michael acceptor for Cys-specific protein bioconjugation. Compared to maleimides, which are ... WebMay 29, 2024 · Although a number of bioconjugation reactions for specific amino acid modification ... J.-R. et al. Chemoselective and photocleavable cysteine modification of peptides and proteins using ... dickins funeral home